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Info.
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Vol.3 - No.2 (2009.06.20)
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Title
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Biosensors on Array Chip by Dual-color Total Internal
Reflection Fluorescence Microscopy
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Authors
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Seong Ho Kang1 & Md. Shahinul Islam1 |
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Institutions
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1Department of Chemistry and Research Institute of Physics and
Chemistry (RINPAC), Chonbuk National University 664-14,
Duckjin-Dong, Duckjin-Gu, Jeonju 561-756, Korea
Correspondence and requests for materials should be addressed
to S.H. Kang (shkang@chonbuk.ac.kr) |
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Abstract
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The noteworthy progress in biosensing technology during the last few decades has paved the way of the real-time detection, rapid screening, characterization and quantification of biological samples with supreme precision. Scientists in this distinct area have made it possible to enhance biochip-based biosensors using optical transducers. This review is meant to provide an overview of biosensors based on nanoarray biochips using polymer-modified glass surfaces and prism type dual-color total internal reflection fluorescence microscopy (TIRFM). It also endeavors to provide information on how the dual-color TIRFM system has made it possible to monitor theinteractions and to detect the colocalization of two different protein molecules (Alexa Fluor® 633 goat anti-rabbit IgG, anti-actin and Alexa Fluor® 488 conjugated actin) at the single-molecular level. |
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Keyword
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Biosensors, Nanoarray biochip, Total internal reflection fluorescence microscopy (TIRFM), Single-molecule detection |
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PDF File
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